Urotensin II in the central nervous system of the frog Rana ridibunda: immunohistochemical localization and biochemical characterization
Identifieur interne : 001944 ( Main/Exploration ); précédent : 001943; suivant : 001945Urotensin II in the central nervous system of the frog Rana ridibunda: immunohistochemical localization and biochemical characterization
Auteurs : Nicolas Chartrel [France] ; J. Michael Conlon [États-Unis] ; Françoise Collin [France] ; Benedicte Braun [France] ; David Waugh [États-Unis] ; Mauro Vallarino [Italie] ; Sabine L. Lahrichi [États-Unis] ; Jean E. Rivier [États-Unis] ; Hubert Vaudry [France]Source :
- Journal of Comparative Neurology [ 0021-9967 ] ; 1996-01-08.
English descriptors
- KwdEn :
Abstract
Urotensin II (UII) is traditionally regarded as a product of the neurosecretory cells in the caudal portion of the spinal cord of jawed fishes. A peptide related to UII has been recently isolated from the frog brain, thereby providing the first evidence that UII is also present in the central nervous system of a tetrapod. In the present study, we have investigated the distribution of UII‐immunoreactive elements in the brain and spinal cord of the frog Rana ridibunda by immunofluorescence using an antiserum directed against the conserved cyclic region of the peptide. Two distinct populations of UII‐immunoreactive perikarya were visualized. The first group of positive neurons was found in the nucleus hypoglossus of the medulla oblongata, which controls two striated muscles of the tongue. The second population of immunoreactive cell bodies was represented by a subset of motoneurons that were particularly abundant in the caudal region of the cord (34% of the motoneuron population). The telencephalon, diencephalon, mesencephalon, and metencephalon were totally devoid of UII‐containing cell bodies but displayed dense networks of UII‐immunoreactive fibers, notably in the thalamus, the tectum, the tegmentum, and the granular layer of the cerebellum. In addition, a dense bundle of long varicose processes projecting rostrocaudally was observed coursing along the ventral surface of the brain from the midtelencephalon to the medulla oblongata. Reversed‐phase high‐performance liquid chromatography analysis of frog brain, medulla oblongata, and spinal cord extracts revealed that, in all three regions, UII‐immunoreactive material eluted as a single peak which exhibited the same retention time as synthetic frog UII. Taken together, these data indicate that UII, in addition to its neuroendocrine functions in fish, is a potential regulatory peptide in the central nervous system of amphibians. © 1996 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/(SICI)1096-9861(19960108)364:2<324::AID-CNE10>3.0.CO;2-P
Affiliations:
- France, Italie, États-Unis
- Californie, Haute-Normandie, Nebraska, Région Normandie
- Mont‐Saint Aignan, Mont‐Saint‐Aignan
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001414
- to stream Istex, to step Curation: 001412
- to stream Istex, to step Checkpoint: 000F38
- to stream Main, to step Merge: 001A50
- to stream Main, to step Curation: 001944
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Urotensin II in the central nervous system of the frog Rana ridibunda: immunohistochemical localization and biochemical characterization</title><author><name sortKey="Chartrel, Nicolas" sort="Chartrel, Nicolas" uniqKey="Chartrel N" first="Nicolas" last="Chartrel">Nicolas Chartrel</name></author><author><name sortKey="Conlon, J Michael" sort="Conlon, J Michael" uniqKey="Conlon J" first="J. Michael" last="Conlon">J. Michael Conlon</name></author><author><name sortKey="Collin, Francoise" sort="Collin, Francoise" uniqKey="Collin F" first="Françoise" last="Collin">Françoise Collin</name></author><author><name sortKey="Braun, Benedicte" sort="Braun, Benedicte" uniqKey="Braun B" first="Benedicte" last="Braun">Benedicte Braun</name></author><author><name sortKey="Waugh, David" sort="Waugh, David" uniqKey="Waugh D" first="David" last="Waugh">David Waugh</name></author><author><name sortKey="Vallarino, Mauro" sort="Vallarino, Mauro" uniqKey="Vallarino M" first="Mauro" last="Vallarino">Mauro Vallarino</name></author><author><name sortKey="Lahrichi, Sabine L" sort="Lahrichi, Sabine L" uniqKey="Lahrichi S" first="Sabine L." last="Lahrichi">Sabine L. Lahrichi</name></author><author><name sortKey="Rivier, Jean E" sort="Rivier, Jean E" uniqKey="Rivier J" first="Jean E." last="Rivier">Jean E. Rivier</name></author><author><name sortKey="Vaudry, Hubert" sort="Vaudry, Hubert" uniqKey="Vaudry H" first="Hubert" last="Vaudry">Hubert Vaudry</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:0FC60A28AEA01B2A55C1216FC4BD01C0453FB282</idno><date when="1996" year="1996">1996</date><idno type="doi">10.1002/(SICI)1096-9861(19960108)364:2<324::AID-CNE10>3.0.CO;2-P</idno><idno type="url">https://api.istex.fr/document/0FC60A28AEA01B2A55C1216FC4BD01C0453FB282/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001414</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001414</idno><idno type="wicri:Area/Istex/Curation">001412</idno><idno type="wicri:Area/Istex/Checkpoint">000F38</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000F38</idno><idno type="wicri:doubleKey">0021-9967:1996:Chartrel N:urotensin:ii:in</idno><idno type="wicri:Area/Main/Merge">001A50</idno><idno type="wicri:Area/Main/Curation">001944</idno><idno type="wicri:Area/Main/Exploration">001944</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Urotensin II in the central nervous system of the frog Rana ridibunda: immunohistochemical localization and biochemical characterization</title><author><name sortKey="Chartrel, Nicolas" sort="Chartrel, Nicolas" uniqKey="Chartrel N" first="Nicolas" last="Chartrel">Nicolas Chartrel</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Mont‐Saint‐Aignan</settlement></placeName></affiliation></author><author><name sortKey="Conlon, J Michael" sort="Conlon, J Michael" uniqKey="Conlon J" first="J. Michael" last="Conlon">J. Michael Conlon</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Nebraska</region></placeName><wicri:cityArea>Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University Medical School, Omaha</wicri:cityArea></affiliation></author><author><name sortKey="Collin, Francoise" sort="Collin, Francoise" uniqKey="Collin F" first="Françoise" last="Collin">Françoise Collin</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Mont‐Saint‐Aignan</settlement></placeName></affiliation></author><author><name sortKey="Braun, Benedicte" sort="Braun, Benedicte" uniqKey="Braun B" first="Benedicte" last="Braun">Benedicte Braun</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Mont‐Saint‐Aignan</settlement></placeName></affiliation></author><author><name sortKey="Waugh, David" sort="Waugh, David" uniqKey="Waugh D" first="David" last="Waugh">David Waugh</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Nebraska</region></placeName><wicri:cityArea>Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University Medical School, Omaha</wicri:cityArea></affiliation></author><author><name sortKey="Vallarino, Mauro" sort="Vallarino, Mauro" uniqKey="Vallarino M" first="Mauro" last="Vallarino">Mauro Vallarino</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Institute of Comparative Anatomy, University of Genova, 16132 Genova</wicri:regionArea><wicri:noRegion>16132 Genova</wicri:noRegion></affiliation></author><author><name sortKey="Lahrichi, Sabine L" sort="Lahrichi, Sabine L" uniqKey="Lahrichi S" first="Sabine L." last="Lahrichi">Sabine L. Lahrichi</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, La Jolla</wicri:cityArea></affiliation></author><author><name sortKey="Rivier, Jean E" sort="Rivier, Jean E" uniqKey="Rivier J" first="Jean E." last="Rivier">Jean E. Rivier</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, La Jolla</wicri:cityArea></affiliation></author><author><name sortKey="Vaudry, Hubert" sort="Vaudry, Hubert" uniqKey="Vaudry H" first="Hubert" last="Vaudry">Hubert Vaudry</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Mont‐Saint‐Aignan</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, 76821 Mont‐Saint Aignan</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Mont‐Saint Aignan</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Comparative Neurology</title><title level="j" type="abbrev">J. Comp. Neurol.</title><idno type="ISSN">0021-9967</idno><idno type="eISSN">1096-9861</idno><imprint><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><date type="published" when="1996-01-08">1996-01-08</date><biblScope unit="volume">364</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="324">324</biblScope><biblScope unit="page" to="339">339</biblScope></imprint><idno type="ISSN">0021-9967</idno></series><idno type="istex">0FC60A28AEA01B2A55C1216FC4BD01C0453FB282</idno><idno type="DOI">10.1002/(SICI)1096-9861(19960108)364:2<324::AID-CNE10>3.0.CO;2-P</idno><idno type="ArticleID">CNE10</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-9967</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>amphibians</term><term>brain</term><term>motoneurons</term><term>neuropeptides</term><term>spinal cord</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Urotensin II (UII) is traditionally regarded as a product of the neurosecretory cells in the caudal portion of the spinal cord of jawed fishes. A peptide related to UII has been recently isolated from the frog brain, thereby providing the first evidence that UII is also present in the central nervous system of a tetrapod. In the present study, we have investigated the distribution of UII‐immunoreactive elements in the brain and spinal cord of the frog Rana ridibunda by immunofluorescence using an antiserum directed against the conserved cyclic region of the peptide. Two distinct populations of UII‐immunoreactive perikarya were visualized. The first group of positive neurons was found in the nucleus hypoglossus of the medulla oblongata, which controls two striated muscles of the tongue. The second population of immunoreactive cell bodies was represented by a subset of motoneurons that were particularly abundant in the caudal region of the cord (34% of the motoneuron population). The telencephalon, diencephalon, mesencephalon, and metencephalon were totally devoid of UII‐containing cell bodies but displayed dense networks of UII‐immunoreactive fibers, notably in the thalamus, the tectum, the tegmentum, and the granular layer of the cerebellum. In addition, a dense bundle of long varicose processes projecting rostrocaudally was observed coursing along the ventral surface of the brain from the midtelencephalon to the medulla oblongata. Reversed‐phase high‐performance liquid chromatography analysis of frog brain, medulla oblongata, and spinal cord extracts revealed that, in all three regions, UII‐immunoreactive material eluted as a single peak which exhibited the same retention time as synthetic frog UII. Taken together, these data indicate that UII, in addition to its neuroendocrine functions in fish, is a potential regulatory peptide in the central nervous system of amphibians. © 1996 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>France</li><li>Italie</li><li>États-Unis</li></country><region><li>Californie</li><li>Haute-Normandie</li><li>Nebraska</li><li>Région Normandie</li></region><settlement><li>Mont‐Saint Aignan</li><li>Mont‐Saint‐Aignan</li></settlement></list><tree><country name="France"><region name="Région Normandie"><name sortKey="Chartrel, Nicolas" sort="Chartrel, Nicolas" uniqKey="Chartrel N" first="Nicolas" last="Chartrel">Nicolas Chartrel</name></region><name sortKey="Braun, Benedicte" sort="Braun, Benedicte" uniqKey="Braun B" first="Benedicte" last="Braun">Benedicte Braun</name><name sortKey="Collin, Francoise" sort="Collin, Francoise" uniqKey="Collin F" first="Françoise" last="Collin">Françoise Collin</name><name sortKey="Vaudry, Hubert" sort="Vaudry, Hubert" uniqKey="Vaudry H" first="Hubert" last="Vaudry">Hubert Vaudry</name><name sortKey="Vaudry, Hubert" sort="Vaudry, Hubert" uniqKey="Vaudry H" first="Hubert" last="Vaudry">Hubert Vaudry</name></country><country name="États-Unis"><region name="Nebraska"><name sortKey="Conlon, J Michael" sort="Conlon, J Michael" uniqKey="Conlon J" first="J. Michael" last="Conlon">J. Michael Conlon</name></region><name sortKey="Lahrichi, Sabine L" sort="Lahrichi, Sabine L" uniqKey="Lahrichi S" first="Sabine L." last="Lahrichi">Sabine L. Lahrichi</name><name sortKey="Rivier, Jean E" sort="Rivier, Jean E" uniqKey="Rivier J" first="Jean E." last="Rivier">Jean E. Rivier</name><name sortKey="Waugh, David" sort="Waugh, David" uniqKey="Waugh D" first="David" last="Waugh">David Waugh</name></country><country name="Italie"><noRegion><name sortKey="Vallarino, Mauro" sort="Vallarino, Mauro" uniqKey="Vallarino M" first="Mauro" last="Vallarino">Mauro Vallarino</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001944 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001944 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:0FC60A28AEA01B2A55C1216FC4BD01C0453FB282
|texte= Urotensin II in the central nervous system of the frog Rana ridibunda: immunohistochemical localization and biochemical characterization
}}
| This area was generated with Dilib version V0.6.27. |  |